Tris-buffered saline or TBS is a buffer solution commonly used in biological research.
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Tris-buffered saline (TBS) is isotonic, notoxic buffer used in some biochemical techniques that is maintain the pH within a relatively narrow range. Tris concentration, that provides the buffering capacity, vary from 10 to 100 mM for a solution labeled as TBS. As TBS is used to emulate physiological conditions (as in animal or human body), the pH value is slightly alkaline, ranging from 7.4 to 8.0. The addition of sodium chloride allows for isotonic (mostly used 150 mM NaCl corresponds to physiological conditions: 0.9% NaCl)
TBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. Please dilute with distilled water and adjust the pH prior to use.
On dilution, the resultant 1x TBS should have a final concentration of 150 mM Tris-Cl, pH 7.6; 150 mM NaCl and a pH of 7.6.
Use: Required for general cytology or use as diluent.
Hazards: Harmless-use normal precautions.
Handling: Exercise normal care
Storage: Store at room temperature
Catalog#: TBS01-1000N, 1000 mL/ea.
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